Journal of Nanomedicine & Nanotechnology

Novel nano-insulin formulation modulates cytokine secretion and remodeling to accelerate diabetic wound healing

2^nd International Conference and Exhibition on Pharmaceutical Nanotechnology & Nanomedicine

March 20 - 21, 2019 | New York, USA

Pawandeep Kaur and Diptiman Choudhury

Thapar Institute of Engineering and Technology, India

Posters & Accepted Abstracts: J Nanomed Nanotechnol

Abstract:

Little is known about insulin’s wound healing capability in normal as well as diabetic conditions. We here report specific interaction of silver nanoparticles (AgNPs) with insulin by making a ~2nm thick coat around the AgNPs and its potent wound healing efficacy. Characterization of the interaction of human insulin with silver nanoparticles showed confirmed alteration of amide-I in insulin whereas amide-II and III remained unaltered. Further, nanoparticles protein interaction kinetics showed spontaneous interaction at physiological temperature with ΔG, ΔS, Ea and Ka values -7.48, 0.076, 3.84Kcal mole-1 and 6x105 s-1 respectively. Insulin capped AgNPs (IAgNPs) showed significant improvement in healing activity in vitro (HEKa cells) and in vivo (Wister Rats) in comparison with the control in both normal and diabetic conditions. The underlying mechanism was attributed to a regulation of the balance between pro (IL-6, TNFα) and anti-inflammatory cytokines (IL-10) at the wound site to promote faster wound remodeling.

Biography :

E-mail: pawan26kaur@gmail.com