Journal of Nanomedicine & Nanotechnology

Niosomal delivery of poorly soluble drugs: A promising approach for the delivery of Amphotericin B against C. albicans

5^th International Conference on Nanomedicine and Drug Delivery

June 28, 2023 | Webinar

Ankita Jain and Mani Shankar Bhattacharyya

CSIR-Institute of Microbial Technology, India

Posters & Accepted Abstracts: J Nanomed Nanotechnol

Abstract:

Vesicular systems have attracted interest in pharmaceutical research and have shown promise as a delivery method for medications with better bioavailability, solubility, and lesser toxicity. Self-emulsifying solid dispersions, liposomes, niosomes, micelles, and nanoparticles are considered a suitable delivery agents for poorly soluble drugs therefore, the non-ionic surfactant-based nano-vesicles known as "niosomes", have several benefits: for e.g., increased stability, biocompatibility, nontoxicity, and economical manufacturing. In this research, we created an amphotericin B-loaded niosomal formulation by employing a biodegradable sophorolipid. Size and morphology of the formed formulations were characterized using dynamic light scattering analysis, transmission electron microscopy, and confocal laser imaging, Zeta potential of the formulations were characterized using Zeta sizer. Entrapment of ampB drug inside the formulation was estimated to be 75±5%. Further the AmpB niosomes exhibited 2-3-fold higher activity compared to the free AmpB against C. albicans. The ampB niosomes are also found to be effective against non albicans such as C.parapsilosis, C. krusei and C. glabrata. More cellular death of the AmpB noisome treated cells were observed compared to the free AmpB treated cells (Through PI staining) under CLSM. This work provides a potential remedy for the medicine amphoterecin B's low solubility and toxicity as well to deal with other fungal diseases.
Recent publications:
1. Verma, N.K., Kumar, S., Basotra, S.D., Jain, A., Vij, M., Prasad, G.S., Bhattacharyya, M.S., 2020. Biocatalytic reduction of prochiral ketones to enantiopure alcohols by novel yeast isolates from unique biodiversity. Biocatal. Agric. Biotechnol. 101547.

Biography :

Ankita Jain possesses expertise in drug and gene delivery using non-ionic surfactant-based nanocarriers. With her proficiency in biosurfactant production and characterization, she has utilized sophorolipid biosurfactants to develop highly efficient liposomal formulations. Miss Jain has also explored the potential of gold nanoparticles by conjugating them with sophorolipid to evaluate their antimicrobial activity. Her contribution to the field extends beyond this as she has been involved in the synthesis of enantiopure naphthyl alcohols using yeast cells as wholecell biocatalysts, a critical step in the production of many pharmaceuticals.