Journal of Nanomedicine & Nanotechnology

New simple way to obtain aqueous fullerene nanodispersions for biomedical applications

4^th International Conference on Nanotek & Expo

December 01-03, 2014 DoubleTree by Hilton Hotel San Francisco Airport, USA

Purgina D D, Bashkatova E N, Khaitov M R and Andreev S M

Accepted Abstracts: J Nanomed Nanotechnol

Abstract:

It is known that fullerene C60 and its derivatives have various biological activities including antioxidant, antibacterial, antiviral and some others. But the biggest problem is the complete insolubility of fullerenes in aqueous media. Different techniques to solubilize С60 have been proposed since 1994; however, most of these are time-consuming and requires the use of toxic solvents and sonication. In this work, a remarkably simple procedure to prepare stabile clear aqueous fullerene C60 solution (nC60) is suggested. The fullerene C60 aqueous solutions (nC60) were prepared by simply mixing the C60 solution in N-methylpyrrolidone (NMP) with de-ionized water followed by exhaustive dialysis against distilled water. Additionally, low-molecular weight, natural substance (L-amino acids, monosaccharides and glycerol) were used as stabilizing agents. The conversion of C60 from the crystalline state to the solution nC60was almost quantitative and one can obtain relatively high concentrations of C60, up to 1 mg/ml, with particle sizes about 100 nm. Their UV-Vis spectra as well as FTIR spectra are characteristic of the species described in the literature previously. The mechanism of formation of aqueous fullerene nano dispersions is still unknown; one can assume that there is a formation ofcharge-transfer complex between C60 and NMP molecules together with partial hydroxylation of C60 nanoparticles. The obtained C60 nanodispersions were stable at least 10-12 months at 8-10?C. The proposed method is promising for the preparation of solutions endo fullerenes, and probably for solubilization of higher fullerenes. The samples of nC60 showed anti-inflammatory activity in mouse models of allergy and atopic dermatitis.

Biography :

Purgina D D graduated from I M Sechenov First Moscow State Medical University in 2013. Currently, she is working at the NRC Institute of Immunology in the Department of Nanobiomedical Technology. She has participated in international conferences on nanotechnology and immunology. She is currently working on her PhD thesis.