Brain Disorders & Therapy

Neuroprotective activity of Aristoteline, an alkaloid isolated from Aristotelia chilensis

Global congress on Neuroscience Psychiatry and Mental disorder

July 03, 2023 | Webinar

Rebeca Perez

University of La Frontera, Chile

Scientific Tracks Abstracts: Brain Disord Ther

Abstract:

Introduction: In Chile, currently 180,000 people suffer from some neurodegenerative disease and it is estimated that in 2050, 626,000 people will have Alzheimer's or another dementia. Neurodegenerative diseases show a decreased cerebral blood flow (FSC), causing a lack of oxygenation of tissues, increasing oxidative stress, which contributes to the progression of neuropathologies by the generation of highly reactive molecules that produce mitochondrial and DNA damage. Aristotelia chilensis is a native chilean tree widely used in folk medicine. Its leaves produce non-iridoid monoterpene indole alkaloids, among them aristoteline showed different pharmacological properties suggesting neuroprotective effects. Objective: To determine the neuroprotective activity of aristoteline, evaluating the vasodilator effect in rat aortic rings and the protective activity against proinflammatory stimuli of hydrogen peroxide (H2O2) in human neuroblastoma SH-SY5Y cells. Methodology: Aristoteline was purified from Aristotelia chilensis leaves and chemically characterized by NMR spectroscopy. The vasodilator effect was evaluated by studying vascular reactivity in rat aortic rings. The effect of aristoteline on the contractile response of phenylephrine was studied by preincubating the rings with aristoteline 10-5 M. In the evaluation of cell protection against H2O2, differentiated SH-SY5Y cells were seeded and cultured for 12 h. The cells were then cultured in serum-free DMEM and pretreated with aristoteline and H2O2 (300 μM) for 24 h. Cell viability was determined by MTS assay. Results: Aristoteline produces relaxation in rat aortic rings with intact, denuded or L-NAME preincubated rings with IC50 4.9x10-5 M; 1.1x10-4 M and 5.8x10-5 M, respectively. Moreover aristoteline restore the cell viability induced by H2O2 at 50 μM. Conclusions: Aristoteline shows vasodilator effects, suggesting that could increase the FSC, moreover aristoteline reduce the cytotoxicity induced by oxidative stress with H2O2 in a neuronal cell model. Acknowledgments: Conicyt- Fapesp Project 201808426-0 and Doctoral Scholarship No 21220534 of ANID-Chile.

Biography :

Rebeca Pérez Collado has a degree in Pharmaceutical Chemistry from the University of Havana, Cuba. He has completed the courses corresponding to the master's degree in Medicine Technology and Control and is currently studying for a doctorate in Molecular Biology and Genetics at the Universidad de La Frontera, in Temuco, Chile. As part of his doctoral research, he is in the Laboratory of Natural Products and Drug Discovery where he conducts research in biomedicine, at the interface of chemistry and biology, with special interest in the isolation, structural determination, and development of bioactive compounds obtained from various natural and synthetic sources.