Agrotechnology

Nanosilver wound dressings for antimicrobial activity

3^rd International Conference on Agriculture & Horticulture

October 27-29, 2014 Hyderabad International Convention Centre, India

Priya Gupta, Geetika Jethra, Radheshyam Sharma, B K Mishra and Sharda Choudhary

Posters: Agrotechnol

Abstract:

Use of Chitin and Nanosilver for the treatment of wounds and burns is increasing in recent years. Due to the small size of the particles, the total surface area of the silver exposed is maximised, resulting in highest possible effect per unit of silver.Chitin membranes with 0, 30, 70, 100ppm nanosilver concentrations were prepared and tested for their antimicrobial activity against Pseudomonas, Staphylococcus, E.Coli and Candida. The killing effect of chitin nanosilver membrane against Pseudomonas with initial count of 105 CFU/ml. Continuous retardation of growth was seen with complete killing with time. At 30ppm nanosilver concentration complete killing was observed after 4hrs, at 50ppm after 3hrs and at 70ppm and 100ppm after 1hr. Chitin membrane also showed some antimicrobial activity as the number of bacteria was less than the number of bacteria in the sample without membrane. The killing effect of chitin nanosilver membrane against Staphlycoccus with initial count of 103-105 CFU/ml, a continuous reduction in growth was observed. The killing effect of chitin nanosilver membrane against Candida with initial count 103CFU/ml, a continuous retardation in the growth was observed with complete killing at 70ppm and 100ppm after 24hrs and 5hrs respectively. The antimicrobial activity against E.Coli, the initial concentration was 104CFU/ml, complete killing was observed after 24hrs at 30ppm and 50ppm, after 4hrs at 70ppm and after 2hrs at 100ppm. The membranes were tested for their fluid absorption and moisture vapour transmission rate. Zone of inhibition with chitin nanosilver membranes was measured to determine the antimicrobial activity and it was observed in all types of bacterial strains i.e., Pseudomonas, Staphylococcus, E.Coli. Increasing in the killing effect was found to be proportional to the concentration of nanosilver. Hence, it was found that membranes with 100ppm nanosilver concentration were most effective.