Journal of Vaccines & Vaccination

mRNA vaccines against SARS-CoV-2 induce comparably low long term IgG Fc galactosylation and sialylation levels but increasing IgG4 responses compared to an adenovirus-based vaccines

6^th International Conference on Vaccines, Immunology and Clinical Trials

March 23-24, 2023 | Webinar

Marc Ehlers

University of Luebeck and University Hospital Schleswig-Holstein, Luebeck, Germany

Scientific Tracks Abstracts: J Vaccines Vaccin

Abstract:

The year 2019 marks the beginning of the Covid-19-pandemic and companies fastly developed new vaccine formats against this life-threatening disease. Our working group is highly interested in the development and function of IgG antibodies after vaccination. In addition to the neutralizing potential of the IgG Fab part, the effector function of IgG antibodies is dependent on the Fc part, which is mediated by the IgG subclass and their type of Fc glycosylation. IgG molecules have a conserved glycosylation site at Asn(N)-297. The complex glycan structures always consist of a core structure that can be further extended by four sugar residues: fucose, bisecting N-acetylglucosamine, galactose, and sialic acid. The IgG subclass and Fc glycosylation pattern influences the interaction with complement, classical FcgammaRs and glycan binding receptors. It has been shown that the adjuvant of a vaccine composition has a high influence on the induced IgG subclass and Fc glycosylation pattern. However, the “adjuvant” potential of the new vaccine compositions against SARS-CoV-2 and the effects of repeated immunizations on the long-term IgG subclass and Fc glycosylation pattern remained unexplored. We analyzed anti-Spike (S) IgG subclasses and glycosylation patterns induced after two immunizations with the newly developed mRNAand adenovirus-based vaccines over time up to day 270 (doi: 10.3389/fimmu.2022.1020844).

Biography :

Marc Ehlers is an immunologist and Professor at the University of Luebeck, Germany. His laboratory is interested in the development and function of antibodies, and in particular of different antibody isotypes and subclasses and their Fc glycosylation patterns, in the context of vaccination, cancer, autoimmunity and allergy.