Agrotechnology

Morphological and molecular diversity analysis in Sesame (Sesamum indicum L.)

2^nd International Conference on Agricultural & Horticultural Sciences

Radisson Blu Plaza Hotel, Hyderabad, India February 03-05, 2014

S Lalitha Kiranmayi, Roja V, Sivaraj N and Sivaramakrishnan S

Posters: Agrotechnol

Abstract:

Twenty three accessions of Sesame ( Sesamum indicum L.) were collected from local areas of Andhra Pradesh for analyzing diversity in the species using morphological and molecular characterization. Morphological characterization of 13 quantitative and 2 qualitative parameters was carried out by correlation studies. Wide variations were observed in flower color, seed color and capsule bearing capacity. The seed yield is showing positive correlation with capsules per plant. Euclidian similarity matrix for morphological data was computed between observable distances and dendrogram which was observed as r=0.79. Cluster analysis revealed two major clusters. Molecular analysis included the study of accessions with 10 SSR markers which revealed 4 polymorphic markers. A total of 14 alleles were detected at the four loci with an average of 3.5 alleles/ locus. The pair wise similarity based on Dice coefficient for all the accessions ranged from 0 to 1 and the correlation coefficient (r) computed between observed distances and dendrogram was 0.77 which indicates a good fit between observed distances and dendrogram. The accessions were genetically and phenotypically found to be diverse. Their genetic distance ranged from 0.1- 0.9 which is wider than their phenotypic distance ranged from 0.2-0.7. When MXCOMP was carried out between phenotypic and genotypic data the correlation coefficient value (r)=0.12 which was showing less congruency. Hence the morphological traits were not influenced by molecular variations in S. indicum .