Journal of Vaccines & Vaccination

Molecular characterization of glucose-6-phosphate dehydrogenase deficiency specific variants in Amhara region, Ethiopia

13^th Annual Congress on Vaccines, Therapeutics & Travel Medicine: Influenza & Infectious diseases

December 01-02, 2016 Atlanta, USA

Yeshwondm Mamuye and Getachew Abebe

Pasteur Institute of Iran, Iran

Posters & Accepted Abstracts: J Vaccines Vaccin

Abstract:

Background: Glucose 6-phosphate dehydrogenase deficiency (G6PDd) is an X-linked hereditary genetic defect, affects 400 million peoples worldwide. Severe clinical manifestation associated with G6PDd (e.g., chronic hemolytic anemia) depends on the type of G6PD molecular variants and exposure to hemolytic triggers (e.g., antimalarial like primaquine). However, scarce studies on G6PDd render the use of primaquine for effective therapeutic treatment of malaria. Objective: To determine the availability and characterize selected molecular variants of G6PDd specific genes among selected populations in malaria endemic area of Amhara region, Ethiopia. Method: Using cross sectional study design a total of 156 dried blood samples were randomly selected from 360 stored samples of national malaria indicator survey of 2011 starting from July 30/2014 to January 30/2015. Polymerase chain reaction and restricted fragment length polymorphism technique was applied to characterize G6PDd variants as G6PD*A, G6PD*A- and/or G6PD*Mediterranean. Binary logistic regression was applied to see association (P<0.05 is significant) among different parameters. Result: Of 156 studied dried blood spot samples, 10 (6.4%) had G6PD genotype available. G6PD*A (100%) was the only genotype characterized, while neither G6PD*A- nor G6PD* Mediterranean genotypes were detected. There was no statistical significant difference between G6PDd and other socio demographic and risk related variables (P>0.05). Conclusion: G6PD*A variant was the only G6PDd genotype detected in this study. G6PD*A variant has almost (90%) the same enzymatic activities with the wild type. Therefore; this result supports the safe use of primaquine, especially the single low dose for transmission interruption of Plasmodium falciparum gametocyte and radical cure of Plasmodium vivax, as a part of malaria elimination toolkit, among selected populations in malaria endemic areas of Amhara region.

Biography :

Email: y_mamuye@yahoo.com