Clinical Microbiology: Open Access

Molecular characterization of cotton infecting Begomovirus(es) and DNA satellites associated with cotton leaf curl disease in Pakistan

7^th Annual Summit on Microbiology: Education, R&D and Market

September 28-29, 2018 | San Antonio, USA

Malik Nawaz Shuja and Muhammad Tahir

National University of Sciences and Technology, Pakistan
Kohat University of Science and Technology, Pakistan

Posters & Accepted Abstracts: Clin Microbiol

Abstract:

Cotton leaf curl disease (CLCuD) has been a considerable encumbrance to the cotton production worldwide including Pakistan. The disease has been caused by a complex of Begomoviruses in association with cotton leaf curl Multan alpha/beta-satellite. Previously, we reported a new strain of cotton infecting Begomovirus from District Layyah, a probable recombinant of Cotton leaf curl Kokhran virus and Cotton leaf curl Multan virus ??? the two predominant species attributed to CLCuD in Pakistan. In this study, symptomatic cotton samples were collected from the cotton belt in Punjab province. Total nucleic acid from leaf tissues was extracted and subjected to PCR. Thirty full-length genomic components were cloned and sequenced, corresponding to Begomovirus and associated DNA alpha-and beta-satellite. Further, a partial dimeric (0.63 mer) DNA-A, (0.55 mer) beta- and (0.9 mer) alphasatellite clones were constructed in a binary vector and used in Agrobacterium-mediated inoculation of Nicotiana benthamiana, N. tabacum, Lycopersicon esculentum, Cucurbita and Cucumis sativus. Leaf curling, deformation and yellowing like symptoms were observed on inoculated plants. Following PCR amplification, the viral DNA, as well as sub-genomic components, were detected in newly emerging non-inoculated leaves. Both molecular and biological studies indicate that this new isolate is a virulent strain of CLCuD and has the potential to aggravate symptomology.

Biography :

E-mail: maliknshuja@yahoo.com