Agrotechnology

Molecular analysis of chilli varieties against anthracnose disease

2^nd International Conference on Agricultural & Horticultural Sciences

Radisson Blu Plaza Hotel, Hyderabad, India February 03-05, 2014

Venkatesham

Posters: Agrotechnol

Abstract:

Pepper fruit anthracnose caused by Colletotrichum capsici is an economically important disease that cause serious yield loss and often post harvesting quality deterioration in many Asian countries. The chilli varieties LCA 235, LCA334 and G-4 characteristically studied and these three varieties are most sustain verities implementation from the present verities by the inoculation methods at various spores suspension level (â??500 spores μl and â??300 spores μl ) chilli anthracnose cause agent Colletotrichum capsici sample collect from different area for different chillies treatments. After inoculation period we can justified by Kochs postulates proof of pathogenicity by the PCR detection from agar culture. The results revealed that these isolates could be differentiated in pathogenecity. These three chilli varieties were treated with different spore suspension of Anthracnose. C.capsici at various inoculation methods. Eg: LCA 235IT 1 /â??500 spores μl, LCA 235 IT 2 /â??500 spores μl LCA 235IT 1 /â??300 spores μl, LCA 235 IT 2 /â??300 spores μl Continued up to G 4 III T 5 /â??500 spores μl and G 4 III T 5 /â??300 spores μl. After inoculation period we can justified by Kochs postulates proof of pathogenicity by the PCR detection from agar culture. The results revealed that these isolates could be differentiated in pathogenicity by the disease score the fruits are scored on 0-10 scale severity for spray inoculation. Although the management and central of Anthrancnose disease are still being extensively researched commercial culture of capsicum annum that are resistant to the pathogen, that cause chilies Anthracnose have not yet been developed. The result of this study revealed that isolates of Colletotrichum capsici were differed from physiological character. Most isolates of C. capsici were expressed the compatible interaction to G4 and LCA334 variety. It can be suggested that this variety were susceptible to all isolate of C. capsici . Other chilli variety LCA235 reacted to most isolates was not distinct. The plant which shows disease resistant or susceptible depends on genotypes of variety. The study on resistance to disease should be based on knowledge of infection, and pathogenicity of the fungus. Even though the result of this study could not be concluded which variety was susceptible or resistant to anthracnose, but it is the basis for further investigation on resistance induction by various plant inducers among these chilli varieties.