Journal of Plant Pathology & Microbiology

Mobile small RNA: Implication for common function during interaction between different hostparasitic plant complexes

8^th World Congress on Plant Genomics and Plant Science

August 10-11, 2018 Osaka, Japan

Subhankar Bera, Kohki Shimizu, Keisuke Tanaka, Shunsuke Yajima, Katsushi Yamaguchi, Shuji Shigenobu and Koh Aoki

Osaka Prefecture University, Japan
Tokyo University of Agriculture, Japan
National Institute for Basic Biology, Japan

Scientific Tracks Abstracts: J Plant Pathol Microbiol

Abstract:

Parasitic plants are grown on hosts through haustorial formation to uptake water and nutrients for their survival and growth. During host-parasitic interaction plant endogenous mRNA and proteins also moved bi-directionally through parasitic interface tissue. Recent studies shown that parasitic plant accumulate miRNA in interphase tissue of host-parasitic complex to control/or regulate host gene through secondary siRNA production. However, there is no direct evidence of host-derived small RNAs moved to long distance tissue of parasite. The purpose of this study is to find out commonly shared long-distance moved small RNAs in both host and parasitic plants which may have functional regulation during and after host-parasitic interaction. We design experiments on (Cuscuta japonica-Glycine max) and (Cuscuta campestris-Arabidopsis thaliana) as different species of parasitic plants grow on different family of host plants. Small RNA-seq analyses and comparison of non-parasitic and parasitic tissue of both parasite and host plants showed that several small RNAs are produced from common gene family of hosts moved to long distance tissue of parasitic plant. Similarly, there are some orthologous genes of different parasitic plant species produced small RNAs from and moved to long distance tissues of hosts. Small RNA candidates target common of orthologous hosts genes also. By stem-loop PCR followed by Sanger sequencing validate small RNAs. Long distance movement was proved by cross-species detection of sRNAs. These results suggest that small RNAs are moved in bidirectional manner to control trans-species gene regulation.

Biography :

Subhankar Bera is pursuing his PhD degree on plant science in Osaka Prefecture University. His PhD work is based on small RNA analyses trace the movement of small RNAs. He is building his experience in research and educational institution. He want's to devote and dedicate himself in dynamic and creative academic interest and to utilize his logical sense, analytical power, potentiality and skills those provide steady growth.

E-mail: subhankar.botany@gmail.com