Journal of Vaccines & Vaccination

Mimotopes selected with neutralizing antibody against multiple subtypes of infl uenza A

International Conference & Exhibition on Vaccines & Vaccination

22-24 Nov 2011 Philadelphia Airport Marriott, USA

Yanwei Zhong, Jiong Cai, Chuanfu Zhang, Lu Liu, Dongqing Zhou, JiaqiHan, Shishu Zhu, Hongfei Zhang, Hongbin Song, Dongping Xu

Scientific Tracks Abstracts: J Vaccines Vaccin

Abstract:

Background: Th e development of novel infl uenza vaccines inducing a broad immune response is an important objective. Th e mimotopes of viruses are considered to be good targets for the vaccine design. Th e aim of this study was to prepare mimotopes against multiple subtypes of infl uenza A and evaluate its immune responses in fl u virus challenge Balb/c mice. Methods: Th e mimotopes of infl uenza A including pandemic H1N1, H3N2, H2N2 and H1N1swine-origin infl uenza virus were screened by peptide phage display libraries , respectively. Th ese mimotopes were engineered in one protein as multi- epitopes in E. coli system and purifi ed by affi nity chromatography with Ni2+-NTA-resin. Balb/c mice were immunized using the multi- mimotopes protein and specifi c antibody responses were analyzed using hemagglutination inhibition (HI) assay an d enzyme-linked immunosorbent assay (ELISA).Th e lung infl ammation level was evaluated by hematoxylin and eosin (HE). Results: linear heptopeptide and dodecapeptide mimotopes were obtained for H2N2 antibody C179, H1N1, H3N2 and swine-origin infl uenza virus antibodies. Th e recombinant multi-mimotopes protein was expressed in Escherichia coli as a 73kDa recombinant fusion protein . Comparing immunized infected groups with unimmunized infected subsets , there was a signifi cant diff erence was observed in the body weight loss and survival rate, the antiserum contained higher HI Ab titer against H1N1 virus , the lung infl ammation level were signifi cantly decreased. Conclusions: phage-displayed mimotopes against multiple subtypes of infl uenza A were accessible to the mouse immune system and triggered a humoral response to H1N1, H3N2, H2N2 and swine-origin infl uenza virus strain. Th e recombinant multi-mimotopes could provide a novel and promising vaccine candidate for the inducing a broad immune response.