Journal of Nanomedicine & Nanotechnology

Microwave synthesis of near infrared type II water-soluble lead sulfide quantum dots for in vivo bio-imaging

17^th International Conference and Exhibition on Nanomedicine and Nanotechnology in Healthcare

November 23-24, 2017 Melbourne, Australia

Jun Chen and Shiyi Chen

Huashan Hospital-Fudan University, China

Posters & Accepted Abstracts: J Nanomed Nanotechnol

Abstract:

Ribonuclease-A (RNase-A) encapsulated PbS quantum dots (RNase-A@PbS Qdots) which emit in the second near-infrared biological window (NIR-II, ca. 1000â��1400 nm) are rapidly synthesized under microwave heating. Photoluminescence (PL) spectra of the Qdots can be tuned across the entire NIR-II range by simply controlling synthesis temperature. The size and morphology of the Qdots are examined by Transmission Electron Microscopy (TEM), Atomic Force Microscopy (AFM) and Dynamic Light Scattering (DLS). Quantum Yield (QY) measurement confirms that the prepared Qdots are one of the brightest water-soluble NIR-II emitters for in vivo imaging. Their high QY (~17.3%) and peak emission at ~1300 nm ensure deep optical penetration to muscle tissues (up to 1.5 cm) and excellent imaging contrast at an extremely low threshold dose of ~5.2 pmol (~1 �¼g) per mouse. Importantly, this protein coated Qdot displays no signs of toxicity toward model neuron, normal and cancer cells in vitro. In addition, the animalâ��s metabolism results in thorough elimination of intravenously injected Qdots from the body within several days via the Reticuloendothelial System (RES), which minimizes potential long-term toxicity in vivo from possible release of lead content. With a combination of attractive properties of high brightness, robust photo-stability and excellent biocompatibility, this new NIR-II emitting Qdot is highly promising in accurate disease screening and diagnostic applications. biochenjun@fudan.edu.cn