Brain Disorders & Therapy

Mechanism of ultrasmall superparamagnetic iron oxide nanoparticles-induced glioblastoma multiforme cytotoxicity: Effects on mitochondrial function

International Conference on Neuro Oncology and Rehabilitation

July 21-22, 2016 Brisbane, Australia

Rosita Tsz-Wai Pang, George Fatseas, Mark E Koina, Sam Adamson, Steven S Eamegdool, Michael W Weible II, Nguyen Pham, Binh T T Pham, Brian S Hawkett and Tailoi Chan-Ling

University of Sydney, Australia
The Canberra Hospital, Australia
Bosch Institute University of Sydney, Australia

Posters & Accepted Abstracts: Brain Disord Ther

Abstract:

This study investigated the in vitro cytotoxic effects of Ultrasmall Superparamagnetic Iron Oxide Nanoparticles (USPIONs) uptake on GBM function. USPIONs with a mean core diameter between 10â??15 nm were loaded to CNS-1 cells cultures at different concentration (10-200 μg/mL) and its cytotoxic effects were assessed in different time-point (2-24 hr). Rat CNS-1 was chosen as our GBM model in this study because it was developed to obtain a histocompatible astrocytoma cell line with infiltrative and growth pattern similar to human gliomas. The uptake of USPIONs was analysed using the JEOL1011 transmission electron microscope (TEM) and the iron quantification was assessed using Graphite Furnace Atomic Absorption Spectrometry (GFAAS). The cell viability and the mitopotential were measured using the MUSE Count & Viability Kit and the MUSE Mitopotential Assay Kit. Bioenergetics was examined using Seahorse Mito Stress Test. TEM showed that USPIONs entered CNS-1 via clatherin coated pits which were then internalized in vacuoles. The biological effects of USPIONs on CNS-1 cell viability and mitopotential were dose and time-dependent. USPIONs at 5-200 μg/mL decreased the cell viability of CNS-1 cells at 12 hr (Control: 100%±0, USPIONs: 74.62%±2.11, P<0.05). USPIONs at 10-200 μg/mL increased the percentage of total depolarized cells at 12 hr (Control: 0.03±0.01, USPIONs: 0.37±0.12, P<0.05). Through these studies, it deepened our understanding of the cytotoxic effects of USPIONs on GBM function.

Biography :

Email: tpan2483@uni.sydney.edu.au