Journal of Food Processing & Technology

Meat identification between buffalo and cattle using 16s rRNA amplification and PCR-RFLP

International Conference and Exhibition on Food Processing & Technology

November 22-24, 2012 Hyderabad International Convention Centre, India

Anjana Sharma, Shiv Kumar, Neha and Deepak Sharma

Posters: J Food Process Technol

Abstract:

Adulteration of meat of one species with another species is a common malpractice in the meat market, however mixing the buffalo meat with cattle meat has an additional religious sting. There are a number of techniques to identify the species from raw meat, however to identify the species from from cooked or processed meat is extremely different.The advances of DNA based techniques has provided us number of powerful techniques to identify the species even from very small samples of cooked/ processed meat. The amplification of mitochondrial genes and their subsequent PCR-RFLP is one of the promising techniques for species identification. In the present study an attempt has been made to differentiate cattle and buffalo on the basis of PCR-RFLP. The steam-cooked meat was prepared based on common adulteration practices that are followed in the local market. Here Buffalo and Cattle mat was mixed to prepared a mixture at 50:50, 75:25, 90:10 and 99:1 proportions. The genomic DNA was isolated from mixed meat.The selective 16S rRNA fragment was amplified a ~590 bp fragment using genomic DNA isolated from mixed meat. The ~590 bp PCR product was digested with the species-specific restriction enzymes. The ~590 bp fragments amplified from the mixed buffalo and cattle meat samples were digested with BsmI. Digestion with BsmI restriction enzyme was expected to produce 2 restriction fragments of 373 bp and 218 bp from buffalo meat and 371 bp, 189 bp and 29 bp fragments from cattle meat.

Biography :

Anjana Sharma has completed her Doctorate in Food Technology in 2010 at Food Science and Technology department, Bundelkhand University, Jhansi and her research topic was ?Qualitative admixing of meat from different livestock species using DNA based methods?. Currently she has completed her ICAR Network project entitled ?Network Project on R & D Support of Process Up-gradation of Indigenous Milk Products from National Dairy Research Institute, Bangalore. This abstract is a part of Ph.D. work.