Journal of Nanomedicine & Nanotechnology

Mannose analogue decorated lipidic nanoparticles for targeted drug delivery to brain glioma

4^th International Conference on Nanotek & Expo

December 01-03, 2014 DoubleTree by Hilton Hotel San Francisco Airport, USA

Indu Singh, Rajan Swami, Wahid Khan and Ramakrishna Sistla

Accepted Abstracts: J Nanomed Nanotechnol

Abstract:

Chemotherapy for brain glioma has been of limited value due to the inability of transport of drug across the blood-brain barrier (BBB) and poor penetration of drug into the tumor. For overcoming these hurdles, surface conjugated lipidic nanoparticles were developed with novel mannose derivative for targeting brain glioma. Lipidic nanoparticle were prepared by solvent emulsification and evaporation process and consequently characterized by various techniques like Differential scanning calorimetery (DSC), Dynamic light scattering (DLS). Drug loaded lipidic nanoparticles were surface modified with mannose derivative using carbodiimide coupling. Conjugation was confirmed using Infrared spectroscopy (IR). Drug encapsulation and in vitro release studies were carried out using HPLC. Conjugated lipidic nanoparticles were found to give sustain drug release as compared to drug solution. The targeting effects were evaluated on the glioma cell lines (U-87 MG). Cell toxicity assay were performed and results were encouraging with remarkable decrease in IC50 values as compared to drug encapsulated unconjugated lipidic nanoparticles and drug control and was further corroborated with cell uptake assay. Drug loaded mannose derivative-conjugated lipidic nanoparticles showed better IC50 and improved cell uptake. Hence, these conjugated lipidic nanopartiles are efficient delivery vehicle to target drugs to brain tumors.

Biography :

Indu Singh has completed her MS from U.P Technical University, India. She was recipient of gold medal during her graduation as well as post graduation. Currently, she is a doctoral student in Department of Pharmaceutics, NIPER-Hyderabad, working under the mentorship and co-mentorship of Dr. Ramakrishna Sistla, Scientist E-II, CSIR-Indian Institute of Chemical Technology, Hyderabad, India and Dr. Wahid Khan, Assistant Professor, National Institute of Pharmaceutical Education & Research (NIPER), Hyderabad, India respectively. She has been working on bioconjugated nanoparticles for brain tumors and other brain diseases like Alzheimers, Parkinson disease etc.