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Magneto-enrichment of genomic calf thymus DNA onto surface functionalized magnetic nanoparticles
11th International Conference and Expo on Nanoscience and Molecular Nanotechnology
October 20-22, 2016 Rome, Italy
A P Tiwari and S H Pawar
D Y Patil University, India
Posters & Accepted Abstracts: J Nanomed Nanotechnol
Abstract:
Maximizing DNA adsorption on magnetic nanoparticles (MNPs) is important for their successful utilization in gene transfer, DNA isolation and bio-analytical applications. This enhancement is typically achieved by BSA @ Fe3O4 and chitosan @silica@ Fe3O4. We demonstrate a novel route for DNA enrichment on BSA @ Fe3O4 and chitosan @silica@ Fe3O4 nanoparticles by applying magnetic field over specific period of time. The results suggested that by applying magnetic field in range of 3-4 minutes, the enrichment of almost 100% genomic DNA could be achieved, and hence giving almost 500th fold enhancement of DNA concentration in real samples. The major force involved and responsible is also discussed and an attempt to calculate the possible force acting on DNAmagnetic nanoparticle complex is also done. The proposed method and the in-depth insight of possible forces involved in magnetic enrichment by magnetic nanoparticles can be beneficial in the designing systems for various biomedical applications.
Biography :
Email: arpitaptiwari@gmail.com