Journal of Stem Cell Research & Therapy

Magnetic hydroxyapatite nanoparticles for augmented differentiation of preosteoblast Cells for bone tissue engineering

7^th International Conference and Expo on Cell and Stem Cell Research

June 28, 2023 | Webinar

Aravinthan Adithan

University of South Florida, USA

Scientific Tracks Abstracts: J Stem Cell Res Ther

Abstract:

Successful materials design for bone-tissue engineering requires an understanding of the composition and structure of native bone tissue, as well as appropriate selection of biomimetic natural or tunable synthetic materials. The inherent magnetism of magnetic nanoparticles influences cellular microenvironments and provide direction in enhancing the osteoconductive, osteoinductive and angiogenic properties. In particular, hydroxyapatite (HAP, Ca10(PO4)6(OH)2) has gained significant interest in orthopedic applications as a major inorganic mineral of native bone. Thus, the present study synthesizes HAP decorated MNPs (MHAP NPs) via the wet chemical co-precipitation method. Synthesized MHAP NPs were evaluated against the preosteoblast MC3T3-E1 cells towards concentrationdependent cytotoxicity, proliferation, morphology, ROS generation, and osteogenic differentiation. The result evidenced that MHAP NPs concentration up to 10 μg/ml was non-toxic even with the time-dependent proliferation studies. As nanoparticle concentration increased, FACS and ROS data showed a significant rise in apoptosis and ROS generation. The MC3T3-E1 cells cocultured with 5 μg/ml MHAP NPs showed significant osteogenic differentiation potential. The MNPs has the potential to deliver magnetically triggered biochemical cues in combination with 3D scaffolds and in vivo evaluation for bone tissue regeneration. Recent publications: 1. Magnetic Hydroxyapatite Composite Nanoparticles for Augmented Differentiation of MC3T3-E1 Cells for Bone Tissue Engineering. Vignesh Krishnamoorthi Kaliannagounder, Mohammad Amjad Hossain, Jong-Hoon Kim, Muthukumar Thangavelu, Aravinthan Adithan*. Marine Drugs 21(2)-85 (2023). 2. (Zn, Ni)-ferrite nanoparticles for promoted osteogenic differentiation of MC3T3-E1 cells. Harshavardhan Mohan, Natesan Karthi, Pavithra Muthukumar Sathya, Vaikundamoorthy Ramalingam, Srivalli Thimmarayan, Mohammad Amjad Hossain, Adithan Aravinthan*, Taeho Shin. Journal of Industrial and Engineering Chemistry 111, 454-463 (2022). 3. Highly efficient visible light driven photocatalytic activity of zinc/ferrite: carbamazepine degradation, mechanism and toxicity assessment. Harshavardhan Mohan, Vaikundamoorthy Ramalingam, Aravinthan Adithan, Karthi Natesan, Kamala- Kannan Seralathan, Taeho Shin. Journal of Hazardous Materials 416; 126209 (2021). 4. IGF-1 facilitates cartilage reconstruction by regulating PI3K/AKT, MAPK, and NF-kB signaling in rabbit osteoarthritis. Mohammad Amjad Hossain, Aravinthan Adithan, Md Jahangir Alam, Spandana Rajendra Kopalli, Bumseok Kim, Chang-Won Kang, Ki-Chul Hwang, Jong-Hoon Kim. Journal of Inflammation Research. 14:3555 (2021). 5. Ginsenoside Rb1 inhibits monoiodoacetate-induced osteoarthritis in postmenopausal rats through prevention of cartilage degradation. Adithan Aravinthan, Mohammad Amjad Hossain, Bumseok Kim, Chang-Won Kang, Nam Soo Kim, Ki-Chul Hwang, Jong-Hoon Kim. Journal of Ginseng Research. 45:287-294 (2021).

Biography :

Aravinthan Adithan has completed his PhD from Jeonbuk National University, South Korea. He is currently working as researcher at the university of South Florida, USA. He has over 50 publications that have been cited over 1800 times, and his publication H-index is 25 and has been serving as reviewer and editorial board member of reputed Journals.