Journal of Nanomedicine & Nanotechnology

Magnetic drug targeting and tumor irradiation by ultrasound enhanced anti-cancer effects of 5-fluorouracil on CT26 tumors in BALB/c Mice

8^th World Medical Nanotechnology Congress & Expo

June 08-09, 2016 Dallas, USA

Ali Shakeri-Zadeh, Samira Hosseini Nami and S Kamran Kamrava

Iran University of Medical Sciences, Iran

Posters & Accepted Abstracts: J Nanomed Nanotechnol

Abstract:

Here, we report a new method to magnetically target the 5-fluorouracil (5-Fu) loaded magnetic poly lactic-co-glycolic acid (PLGA) nanocapsules towards CT26 colon tumor model in BALB/c mice. Additionally, we ultrasonicated the tumors impregnated by nanocapsules with the goal of aiding in magnetic drug targeting (MDT) procedure. Newly synthesized 5-Fu-loaded PLGA magnetic nanocapsules were synthesized and characterized. Various treatment modalities with the use of nanocapsules, magnetic field, and ultrasound were applied to the tumors and appropriate controls were considered. To analyze the nanocapsules distribution within the CT26 tumor, Magnetic Resonance Imaging (MRI) and Prussian Blue (PB) staining were performed. Anti-tumor and pro-apoptotic effects of each treatment modality on CT26 tumors were investigated. The effective diameter of nanocapsules was approximately 70 nm. Histological staining of the tumor tissue with PB as well as MRI showed a broad distribution of magnetic nanocapsules within the tumor and confirmed the targeting of nanocapsules to the tumors. Anti-tumor studies demonstrated that the combination of nanocapsules-MDT-ultrasound effectively inhibits the growth of CT26 tumors compared with injection of 5-Fu alone (P<0.01). The present study exhibits potentials of the newly synthesized magnetic nanocapsule and suggests the combination of MDT and ultrasound might help this new nanotechnology-based cancer chemotherapy agent in vivo.

Biography :

Email: shakeriz@iums.ac.ir shakeri2005@gmail.com