Journal of Bioequivalence & Bioavailability

Liquid chromatographic method for the determination of Cefditoren Pivoxil in pharmaceutical formulations

3^rd World Congress Bioavailability & Bioequivalence

March 26-28, 2012 Marriott Hotel & Convention Centre, Hyderabad, India

Myneni. Harika, M. Mathrusri Annapurna, M.S.L. Sindhu and L. Srinivas

Posters: J Bioequiv Availab

Abstract:

A n isocratic RP-HPLC method was proposed for the determination of Cefditoren pivoxil in pharmaceutical formulations (Tablets). Cefditoren is used to treat uncomplicated skin and skin structure infections, community-acquired pneumonia, acute bacterial exacerbation of chronic bronchitis (ABECB), pharyngitis, and tonsillitis. Cefditoren pivoxil is a third-generation semi-synthetic cephalosporin antibiotic for oral administration. Isocratic elution was performed using phosphate buffer and acetonitrile as mobile phase. The overall run time was 10 min. and the flow rate of the mobile phase was 1.0 mL/min. with UV detection at 220 nm. 20 μL of sample was injected into the HPLC system. In the present work chromatographic separation was achieved by using a C-18 (250mm ? 4.6mm i.d., 5 μm particle size) column of Shimadzu Model CBM-20A/20 Alite, equipped with SPD M20A prominence photodiode array detector, maintained at 25?C. Linearity was observed in the concentration range of 0.1?200 μg/mL (R 2 = 0.9998) and the method was validated as per ICH guidelines. The RSD for intra-day and inter-day precision were found to be less than 2 %. The percentage recovery was in good agreement with the labeled amount in the pharmaceutical formulations and the method is simple, precise, accurate and robust for the determination of Cefditoren pivoxil.