Journal of Bacteriology & Parasitology

Lactobacillus plantarum and EGCG in a synbox: An effective intervention for alcohol induced endotoxin mediated liver disease

7^th World Congress on Microbiology

November 28-29, 2016 Valencia, Spain

Praveen Rishi

Panjab University, India

Posters & Accepted Abstracts: J Bacteriol Parasitol

Abstract:

Alcohol abuse can cause substantial liver insufficiencies leading to significant mortality worldwide, regardless of the available therapeutic options. Recently, we have evaluated the use of alternative agents like Epigallocatechin gallate (EGCG) and L. plantarum, to control ALD. The prebiotic property of EGCG for enhancing the growth of L. plantarum was evaluated. Based on this property a coupled formulation consisting of both EGCG and L. plantarum was developed by encapsulating these agents in Caalginate beads to achieve enhanced biological effects. The formulation was extensively characterized and evaluated for its enhanced in vivo efficiency. Effect of EGCG to enhance the growth of L. plantarum was significantly better than inulin. Combination beads lowered serum transaminases and blood alcohol levels. Alcohol fed rats elicited raised plasma endotoxin levels, attributable to the disrupted intestinal permeability, confirmed by lactulose-mannitol test using HPTLC. Transcription studies for TLR-4 receptor mediated signaling molecules (MyD88, CD14, MD2) revealed modulation in the expression of these molecules, resulting in the down-regulation of NF-κB in rats treated with combination beads. Expression of pro-inflammatory cytokines i.e., TNF-α and IL- 12β/40 subunit, COX-2 in addition to the levels of antioxidants, oxidants and micronuclei formation also assumed normal levels. Histo-architecture depicted normal liver and intestine in rats treated with co-encapsulated beads whereas severely distorted histology was observed for respective tissues in alcoholic rats. Thus, the formulation of L. plantarum with EGCG in a synbox can be a promising therapeutic option ensuring enhanced bioperformance against ALD. To the best of our knowledge, these findings are being reported for the first time.

Biography :

Email: rishipraveen@yahoo.com