Journal of Microbial & Biochemical Technology

Isolation, identification and characterization of two co-metabolic biodegradation of linear alkylbenzene sulfonate strains

International Conference and Expo on Water Microbiology & Novel Technologies

July 18-19, 2016 Chicago, USA

Zhilin Ran, Wen-de Tian and Miao-Miao Sha

Shenzhen Institute of Information Technology, China

Posters & Accepted Abstracts: J Microb Biochem Technol

Abstract:

Two bacterial strains which could degrade linear alkylbenzene sulfonate (LAS) by co-metabolism mechanism, named as L-2 and L-15 were isolated from the activated sludge of aeration tank and the secondary sedimentation pool in Shenzhen sewage treatment plant. Based on physiology and 16S rRNA gene sequence analysis, L-2 was determined to Klebsiella sp., while L-15 was identified as Enterobacter sp. The degradation rates were only 1.1% and 5.9% when they utilized LAS as the sole carbon and energy source. The degradation rate of LAS (50 mg/L) by L-2 was up to 94.2% when glucose was chosen as growth substrate under the conditions as: 30o C, pH 7.5, the mass ratio of glucose and LAS 20:1. The degradation rate of LAS (50 mg/L) by L-15 was up to 92.2% under the conditions as: 30 oC, pH 7.5, the mass ratio of glucose and LAS 16:1. Above results demonstrated that the two bacterial strains could degrade LAS in waste water effectively by co-metabolism mechanism.

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