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Isolation and characterisation of PHA-producing bacteria from the environment
2nd World Congress on Biopolymers
August 04-05, 2016 Manchester, UK
Aisha Alwuhaib
University of Portsmouth, UK
Posters & Accepted Abstracts: J Adv Chem Eng
Abstract:
Polyhydroxyalkanoates (PHAs) are biopolymers that are of interest because they can be used to produce bio-plastics. They are produced by many types of bacteria when environmental conditions are rich in carbon but poor in nitrogen. In this study, we describe the occurrence of PHA-producing bacteria from waste water and composts. Environmental samples were collected from a wastewater treatment plant at Petersfield and from garden compost. Bacterial isolates were recovered after growth on a range of media, including M9 minimal medium, minimal salt medium (MSM) and Luria-Bertani (LB). The minimal media contained a low nitrogen, high carbon ratio. PHA production was initially tested after staining with Nile Blue. From the isolates recovered from the waste water, 40.4% were capable of producing PHAs, whereas 44% of the isolates recovered from compost were able to produce PHAs. rRNA genes were amplified from the isolates and BLAST homology matches identified that the bacteria came from the following genera: Alcaligenes, Aeromonas, Arthrobacter, Bacillus, Pseudomonas, Citrobacter, Acinetobacter, Klebsiella and Raoultella. The presence of a PHA synthase gene (phaC) was confirmed for 23 strains by PCR amplification using primers phaCF1BO and phaCR2BO. However, some strains did not amplify for the phaC gene although they gave a positive staining reaction with Nile Blue. The nature of these strains is being further investigated using a biochip using probes designed as part of this study.
Biography :
Aisha Alwuhaib is a Lab Scientist working at Arabian Oil Company (Saudi Aramco) in Dhahran, Saudi Arabia from 2008-2013. Currently she is a PhD student at the University of Portsmouth studying Molecular Microbiology.
Email: aisha.alwuhaib@port.ac.uk