Journal of Microbial & Biochemical Technology

In-vitro inhibition of BVDV as surrogate model for HCV using novel gold nanoparticles

World Congress and Expo on Applied Microbiology

August 18-20, 2015 Frankfurt, Germany

M. El-gaffary1, Ola M. El-Borady2, M.M.Bashandy1, A.R.Ahmed1

1Faculty of Veterinary medicine, Cairo University, Egypt 2Chemistry Department, Modern Science & Arts University, October city, Egypt

Scientific Tracks Abstracts: J Microb Biochem Technol

Abstract:

In this study, we evaluated in-vitro cytotoxic effect and antiviral properties of gold nanoparticles, which are previously reported to possess in-vitro antiviral properties against HIV and multi strains of influenza virus. To investigate the antiviral activity of gold nanoparticles against cytopathic strain (NADL) bovine viral diarrhea virus (BVDV): citrated gold nanoparticles of 7�±2 nm were prepared and PEG functionalized. Evaluation of the cytotoxicity of prepared gold nanoparticles did not show toxic effects to MDBK cells with concentration of 2 and 4 ppm. Afterward the antiviral activity of nanoparticles was evaluated by the inhibition of the cytopathic effect on infected MDBK cells by means of (MTT) based colorimetric assay and was found that 4 PPM is the optimum concentration for virus inhibition. The results of the in- vitro antiviral activity and cytotoxicity showed that prepared gold nanoparticles has limited in-vitro toxic effect at concentration of 4 PPM also has strong affinity to BVD virus and reasonable inhibitory effect on BVDV.

Biography :

Mostafa El-gaffary has completed his MVSc 2010 from Cairo University and his PHD from Cairo University Faculty of Veterinary Medicine. He is lecturer and director of Clinical pathology Lab in his Faculty; he was Trainer for postgraduate student on Biomedical application of Nanotechnology, Molecular biology and Immunology at Biotechnology center for research located in his Faculty2007 – 2014.