Senthil Kumar Subramanian, V JeyRaghashri, K Jeyalakshmi and Kumar Subramanian
Bharathidasan University, India
University of Witswater, South Africa
Posters & Accepted Abstracts: J Nanomed Nanotechnol
Silver nanoparticles were successfully prepared by eco-friendly and cost effective aerial roots of Rhaphidophora aurea (Linden ex Andre) extract which act as a reducing agent by green synthesis method. The visual color change from colorless to yellow to brown was helpful in confirmation of AgNPs formation. UV Visible (UV-vis) spectroscopy was used to follow the gradual formation of NPs and power X-ray diffraction (XRD) confirmed the face centre cubic crystal structures in software in JANA2006, Edit+3. XRD studies revealed a gradual crystal-lite size reduction at different molar ratios of extract silver salt. The crystallite size was calcu-lated using Debye-Schererâ??s formula and found to be in the range 30 nm. Morphology and characterization of AgNPs was done by using FESEM, HRTEM, FTIR and EDAX tech-niques. FESEM and HRTEM analysis showed that the morphology of the obtained AgNPs was spherical shaped which can be used in variety of biomedical and pharmaceutical applica-tions. The antibacterial actives and mechanism of silver nanoparticles are examined using Escherichia coli, Streptococcus mutants, Bacillus subtilis and Pseudomonas aeruginosa re-spectively by analyzing the growth permeability and morphology of the bacterial cells follow-ing with AgNPs.
Email: senthil.phy10@gmail.com