Clinical Microbiology: Open Access

Investigation and characterization of an antibiotic resistance gene in bacteria identified from deep surface

Joint Event on 17^th International Conference on Pharmaceutical Microbiology and Biotechnology & 23^rd Edition of International Conference on Immunology and Infectious Diseases

April 29-30, 2019 London, UK

Nurcan Vardar Yel

Altinbas University, Turkey

Posters & Accepted Abstracts: Clin Microbiol

Abstract:

The spread of antibiotic resistance among microorganisms will constitute a global threat for us in the near future. To minimize this problem, it is necessary to obtain information on new antibiotic resistance genes from natural environments. As a result of our studies, a beta-lactamase gene was isolated from terrestrial underground environment for the first time by using metagenomics and direct cloning strategy. A total of 10 plasmid DNAs containing resistance gene insertions were randomly selected and sequenced with universal primers. The bacterial ribosomal gene sequences we obtained from sequencing were analyzed using the National Local Biology and Information Center (NCBI) website, which uses existing DNA databases, using the Basic Local Alignment Search Tool (BLAST) function. It has been found that the germs present in this bacterial population are very similar to Bacillus spp. The nucleotide sequences generated for the 16S rRNA genes were deposited at GenBank. Most similar to penicillinase was obtained from Bacillus licheniformis. The resistance gene was confirmed by sub-cloning and the minimum inhibitory concentration values were measured against several test betalactam antibiotics. Different isolates were tested for growth with antibiotics kanamycin (100 μg/ml), ampicillin (100 μg/ml), rifampicin (100 μg/ml), neomycin (50 μg/ml) or tetracycline (10 μg/ml) at 37°C for 24 h. This study extends information about resistance to antimicrobials that can help to minimize future threats.

Biography :

E-mail: nurcan.vardar@altinbas.edu.tr