Agrotechnology

Influence of various compounds on media sterilization in sugarcane meristem tip culture

3^rd International Conference on Agriculture & Horticulture

October 27-29, 2014 Hyderabad International Convention Centre, India

Adilakshmi D, Charumati M, Appalaswamy A, Jayachandra K and Bebi P

Accepted Abstracts: Agrotechnol

Abstract:

Sugarcane is negatively affected by diseases largely due to its vegetative propagation through the use of stem cuttings (setts). Quality seed assumes a great significance in any crop and so it is in sugarcane. Micro propagation is one of the most important techniques for commercial cloning of plants. Despite several clear advantages of in vitro culture techniques, some problems persist and do not satisfy the commercial interests, particularly the high costs of implementation and technical maintenance. In this context, one of the main problems is high energy expenditure and time taken for the preparation of material during the autoclaving phase of culture medium (Macek et al., 1995). Thus the present investigation was taken to screen out the best concentrations of chemical sterilants (HgCl2, NaOCl, H2O2 and AgNO3), fungicides (Carbendazim, Propiconazole, Hexaconazole and Tebuconazole) and natural extracts (aqueous extracts of Morinda citrifolia leaf, Azadirachta indica leaf and Allium sativum bulb) on the in vitro developed shoots of sugarcane using Meristem tip culture. Fresh tops of commercial sugarcane variety grown at the research farm of Regional Agricultural Research Station (RARS), Anakapalle, Andhra Pradesh, India were collected. The data on survival (%), mean number of shoots per culture and mean shoot length (cm) in MS media supplemented with BAP (0.2mg/l), KN (0.1 mg/l) and commercial sugar (27g/l) on media sterilization with various concentrations of chemical sterilants like Mercuric chloride (HgCl2), Sodium hypochlorite (NaOCl), Hydrogen peroxide (H2O2) and Silver Nitrate (AgNO3) indicated that maximum survival (%), mean number of shoots per culture and shoot length of in vitro meristem tip cultures of sugarcane was recorded in 0.100 g/l NaOCl (92.07%, 12.02 and 12.49 cm). The data on the effect of MS media supplemented with BAP (0.2 mg/l), KN (0.1 mg/l) and commercial sugar (27 g/l) on media sterilization with various concentrations of Fungicides viz., Carbendazim, Propiconazole, Hexaconazole and Tebuconazole on survival (%), mean number of shoots per culture and mean shoot length (cm) indicated that, the maximum survival (%), mean number of shoots per culture and mean shoot length (cm) were maximum in carbendazim at 200 g/l (44.75, 5.43 and 6.12). The data on the effect of MS media supplemented with BAP (0.2mg/l), KN (0.1mg/l) and commercial sugar (27g/l) on media sterilization with various concentrations of Natural aqueous leaf extract of Morinda citrifolia, leaf extract of Azadirachta indica and bulb extract of Allium sativum on survival (%), mean number of shoots per culture and mean shoot length (cm) indicated that, maximum survival (%), mean number of shoots per culture and mean shoot length (cm) of in vitro meristem tip cultures of sugarcane was observed in aqueous extract of Morinda citrifolia leaf at 4.000 ml/l (64.78%, 7.48 and 7.55 cm). Among various alternatives for reducing cost in biofactories or commercial plant production laboratories, using NaOCl is a good alternative to replace the autoclaving procedure.