Journal of Advanced Chemical Engineering

In vitro test of antiwrinkle nanoemultion preparate of extract Centella asiatica (Centella asiatica (L) Urb) and ginger (Zingiber officinale Rosc.)

5^th World Congress on Chemical Engineering and Catalysis

August 28-29, 2018 | Paris, France

Dewi Sondari, Silvester Tursiloadi , Tun Tedja Irawadi and Dwi Setyaningsih

Research Center for Chemistry- Indonesian Institute of Sciencies, Indonesia
Bogor Agricultural University, Indonesia

Posters & Accepted Abstracts: J Adv Chem Eng

Abstract:

Preparation of nanoemulsion preparate from the extract of Centella asiatica and ginger is intended to know its activity as antiwrinkle agent by inhibiting elastase and collagenase enzymes. The extraction method of Centella asiatica and ginger is by using the supercritical CO2 extract fluid. Yield of asiaticoside content and total triterpenoid and determination of compound content in Centella asiatica extract was analysed by High Performance Liquid Chromatography (HPLC). Antioxidant activity was measured using a 1,2-diphenyl-2-picrylhydrazyl (DPPH) radical damping test and identified ginger extract components using Gas Chromathography Mass Spectrometry (GC-MS). The inhibitory effect on wrinkling was done by measuring the inhibition of elastase and collagenase enzymes. Based on chromatogram profile of HPLC, showed that Centella asiatica (L) Urb contained the largest asiaticoside compound 4.468 mg/mL, rendement of crude extract was 9.23±0.015 mg/g and total triterpenoid was 34.90±0.084 mg/g extracted at 27 MPa pressure and 50oC temperature with time 4 hours extraction. Results of antioxidant activity with highest DPPH was 63.66±0.181 ?g/mL in ginger extract. The components of ginger extract consisting of volatile oil components is mainly of sesquiterpenes hydrocarbons, i.e., ?-zingiberene (1.07%), ?- sesquiphellandrene (1.08%), ?-curcumene (0.88%), ?-farnesene (0.5%), and ?-bisabolene (1.22%). Components contained in oxygenated monoterpene are eucalyptol, ?-linalool, endo-borneol, terpinen-4- ol, ?- terpineol, citronellol, ?-citral, geraniol, and ?-citral. The monoterpene hydrocarbon component is camphene hydrate, and the oxygenated monoterpene ester component is L-bornyl acetate (0.29%) and geranyl acetate (0.55%). The oleoresin compounds (non volatile oils) contained in the gingerbread extract are gingerol consisting of 6-gingerol (5.76%) and [6] -paradol (0.38%). In addition to the above-mentioned compounds, aldehydes aliphatic compounds (hexanal, octanal, decanal) and other compound components are present in ginger extract (bis (2-ethylhexyl) phthalate and bioctyl terephthalate).

Biography :

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