Journal of Nanomedicine & Nanotechnology

In vitro cytotoxicology effects of zinc oxide flower-like nanostructures in normal and cancer cells

11^th International Conference and Expo on Nanoscience and Molecular Nanotechnology

October 20-22, 2016 Rome, Italy

I M M Paino, F J Goncalves, F L de Souza and V Zucolotto

University of São Paulo, São Carlos, Brazil
Federal University of ABC, Brazil

Posters & Accepted Abstracts: J Nanomed Nanotechnol

Abstract:

Introduction: Metal oxide nanoparticles, including zinc oxide, are versatile platforms for biomedical applications and therapeutic intervention. Zinc oxide nanoparticles (ZnO Nps) are frequently used in consumer products, such as textiles, cosmetics, antibacterial products and they have been described as important for their excellent biomedical applications, such as cancer therapy. ZnO flowerlike nanostructures have been reported as a new therapeutic alternative for cardiovascular diseases. However, there are very few studies conducted on safety, toxicology and exposure of ZnO nanostructures to normal and cancer cells. In this study, we investigate the in vitro cytotoxicology effects of ZnO flower-like nanostructures in human cervical carcinoma (HeLa) cells and normal murine fibroblast (L929) cells. Methods: Apoptosis and necrosis processes were performed using Annexin V-FITC apoptosis BD® kit, production of reactive oxygen species (ROS), and the cellular uptake by side-scattered light (SSC) by flow cytometry (FACSCalibur, BD®, USA). Cells were incubated at 0.1, 1.0 and 10.0 gmL-1 of ZnO flower-like nanostructures synthetized at different times (30 min, 2 h and 4 h) for 24 h and exposure at 37oC, 5% CO2 atmosphere. Results: All results were performed as the mean±standard deviation of 3 independent experiments. Data were submitted to one-way analysis of variance (ANOVA) and the post-hoc Tukey test considering p<0.05. Discussion/Conclusion: The cytotoxic response (p<0.05) observed in normal and cancer cells showed that the ZnO nanostructures exhibit an enhanced toxicology effects in HeLa cancer cells. This ability may be of clinical interest due the effectiveness of ZnO flowerlike nanostructures in cancer therapy.

Biography :

Email: iedammp@hotmail.com