Oral Health and Dental Management

In vitro assessment of endodontic medicaments against E. faecalis and C. albicans

2^nd International Conference and Exhibition on Dental & Oral Health

April 21-23, 2014 Crown Plaza Dubai, UAE

Bajrami D, Hoxha V, Stavileci M and Omeragiq Sh

Accepted Abstracts: Oral Health Dent Manag

Abstract:

Aim: The aim of this study was to evaluate the antimicrobial effect of calcium hydroxide paste, Ledermix R Paste and Klorhexidine gel. Material and Methods: The antimicrobial efficacy of the tested substances was evaluated using the agar diffusion test, against Enterococcus faecalis (ATCC 29212) and Candida albicans (ATCCC 10231) with 0. 5 McFarland. In the 5. 5 mm diameter discs (Liofilchem - blank disc) each medicament was applied and incubated at 37oC/24h. The experiment was done in five replicates for each group. Kruskal-Wallis statistical test was used to evaluate the differences between the susceptibility of individual microbial species to the endodontic irrigants. Significance level was set at 5%. Results:The largest growth inhibition zones were produced when the test bacteria were in contact with 2% chlorhexidinegluconate gel, being significantly different from zones produced by Calcium hydroxide paste and Ledermix. This difference was significant for E. Faecalis (KW=6:49, P <0. 05) and C. Albicans (KW=7. 26, P <0. 05). The results of this study indicate that, as far as its antimicrobial properties are concerned, chlorhexidine gel has a great potential to be used as an endodontic auxiliary chemical substance.