Journal of Food Processing & Technology

In vitro antioxidant, anticancer properties and probiotic characteristics of selected lactic acid bacteria strains

21^st International Conference on Food Technology & Processing

October 04-06, 2018 | London, UK

M G Shehata, Marwa M Abu Serie, Nourhan M Abd El Aziz and S A El Sohaimy

Arid Lands Cultivation Research Institute – SRTA-City, Egypt
Genetic Engineering and Biotechnology Research Institute – SRTA-City, Egypt

Posters & Accepted Abstracts: J Food Process Technol

Abstract:

Probiotic strains can potentially be used as bio-preservatives and functional food supplements. Eight Lactic Acid Bacteria strains (LAB) Lactobacillus brevis NRRL B-4527; Streptococcus thermophilus BLM 58; Pediococcus acidilactici ATCC 8042; Lactobacillus rhamnosus CCUG 1452; Lactobacillus curvatus ATCC 51436; Lactococcus lactis sub sp., Lactis DSM 20481; Lactobacillus plantarum DMSZ 20079 and Lactobacillus plantarum TF103 were selected to screen the antioxidant, anticancer potential and probiotic properties. LAB strains exhibited good probiotic, antioxidant properties and showed antagonistic activity against food borne pathogens (Bacillus subtilis DB 100 host; Candida albicans ATCCMYA-2876; Clostridium botulinum ATCC 3584; Escherichia coli BA 12296; Klebsiella pneumoniae ATCC12296; Salmonella senftenberg ATCC 8400 and Staphylococcus aureus NCTC 10788). Further, in vitro probiotic properties of eight strains displayed excellent acid tolerance, bile tolerance, simulated gastrointestinal juice tolerance, in vitro adhesion ability for HT-29 cell line. The antioxidant effect of intracellular and cell free extract of lactic acid bacteria strains was evaluated by various antioxidant assays namely, resistance to hydrogen peroxide, DPPH radical scavenging, ABTS radical scavenging and Hydroxyl Radical Scavenging (HRS). The results showed that intracellular and cell-free supernatant of S. thermophilus BLM 58, L. lactis sub sp., lactis DSM 20481, P. acidilactici ATCC 8042, L. brevis NRRL B-4527 strains possess excellent antioxidant capacity. The intracellular of S. thermophilus BLM 58 and P. acidilactici ATCC 8042 also showed excellent anticancer activity against Caco-2, MCF-7, HepG-2 and PC-3. Antioxidative property of selected lactic acid bacteria strains would be useful in the functional food manufacturing industry. They could beneficially affect the consumer by providing dietary source of antioxidants.

Biography :

E-mail: gamalsng@gmail.com