Journal of Nanomedicine & Nanotechnology

Implementation of optical biosensors based on silver nanoparticles for the diagnostics of misfolding protein deposit diseases

4^th International Conference on Nanotek & Expo

December 01-03, 2014 DoubleTree by Hilton Hotel San Francisco Airport, USA

Agata Dziwinska

Accepted Abstracts: J Nanomed Nanotechnol

Abstract:

In several different forms of malignant monoclonal gammapathy immunoglobulins free light chains may be produced in high rate of several grams/24 h. This overproduction in some patients is asymptomatic while in others leads to wide spectrum of protein deposition diseases. In AL amyloidosis light chain form organized extracellular fibrils. In other pathologies deposits are amorphous or crystalline. In Fanconi syndrome intracellular crystalline structures impair kidney tubular structures. Until now there is no laboratory test estimating susceptibility of LC for aggregation. Aim of this research is to elaborate optical biosensor based on silver nanoparticles (SNPs) and afterwards obtain its functionality for clinical applications in order to improve the diagnostics of deposit diseases. In view of the alarming increase in frequency of those potentially lethal ailments among older generation, our project responds to global necessity of effective techniques allowing for unequivocal diagnosis. SNPs were synthesized by reduction of silver ions with sodium borohydride. Free light chains have single cysteine group which theoretically should facilitate simple covalent attachment on the surface of silver. In practice this sulfhydryl requires activation with reducing agent. Aggregation of SNPs induced by polyclonal antibodies was demonstrated by change in color of solution from yellow to orange. Furthermore, a characteristic red-shift was detected during the analysis of absorption spectra. Direct optical measurements by means of dark field microscope allowed visualizing dynamics of the aggregation process. By confirming simplicity of SNPs? synthesis and satisfying availability of detection methods we proved previously assumed usefulness of SNPs for creating a reliable nanobiosensor.

Biography :

Agata Dziwinska is in the final year Majoring in Laboratory Medicine at Jagiellonian University. As an active member of the Laboratory Diagnosticians Scientific Society within the Department of Medical Diagnostics, he is currently conducting a research on silver nanoparticles. He has already participated in several international students? conferences such as: International Students? Medical Congress Kosice and Medical Students? Conference in Cracow.