Journal of Stem Cell Research & Therapy

Immunogenicity and therapeutic effects of Rv1419 DNA vaccine from Mycobacterium tuberculosis

4^th International Conference and Exhibition on Cell & Gene Therapy

August 10-12, 2015 London, UK

Yan Liang, Xiaoyan Zhang, Li Xiao, Xuejuan Bai, Xiaomei Wang, Junxian Zhang, Yourong Yang , Jinying Song, Ni Li and Xueqiong Wu

Posters-Accepted Abstracts: J Stem Cell Res Ther

Abstract:

The situation of tuberculosis (TB) is very severe in China. New therapeutic agents and regimens to treat tuberculosis
are urgently needed. In this study, a DNA vaccine expressing Mycobacterium tuberculosis (MTB) Rv1419 antigen was
constructed and evaluated. The mice were immunized intramuscularly three times at two-week intervals with saline, plasmid
vector pVAX1, M. vaccae, Ag85A DNA or Rv1419 DNA. Three weeks after the last immunization, higher levels of IFN-γ were
observed in the mice in the Rv1419 DNA group in stimulated spleen lymphocyte cultured supernatant, and a higher ratio of
Th1/Th2 cells in whole blood was observed, suggesting a predominant Th1 immune response. Compared with saline group,
injection of MTB-infected mice with Rv1419 DNA reduced live bacterial loads found in lungs and livers all by 0.41 log10. The
pathological changes in the lungs of DNA vaccine treated mice were less, with more limited lesions. These results suggest that
Rv1419 DNA was effective for the treatment of TB, significantly increasing the Th1 cellular immune response, and inhibiting
the growth of MTB. Rv1419 DNA can be used as a new candidate DNA vaccine against TB.

Biography :

Yan Liang, MD, PhD is Associate Professor of Army Tuberculosis Prevention and Control Key Laboratory, Beijing Key Laboratory of New Techniques of Tuberculosis
Diagnosis and Treatment, the Institute of Tuberculosis Research, the 309th Hospital of Chinese PLA, Beijing 100091, China. Research areas are New TB vaccines
and rapid diagnostic techniques of tuberculosis.