Journal of Microbial & Biochemical Technology

Identification of thermophilic guar degradation bacteria and the applications in hydraulic fracturing

International Conference and Summit on Industrial & Pharmaceutical Microbiology

October 17-18, 2016 Kuala Lumpur, Malaysia

Guanglun Lei, Xin Ma, Zhihui Wang and Chuanjin Yao

China University of Petroleum, China

Posters & Accepted Abstracts: J Microb Biochem Technol

Abstract:

Guar as the most popular thickener in the hydraulic fracturing increases the viscosity of fluids. Hydraulic fracturing process in oil and gas field is that: High viscosity fracturing fluid with solid proppant is pumped into oil or gas reservoir to obtain the fractures which are effective flow channels of oil and gas. Then breaker will be used to break the gel to reduce the viscosity of fluid to be close to the viscosity of water and flow back. Finally, it is necessary to clean the residue of gel in the formation to avoid blocking. In this paper, we isolate and identify some strains of thermophilic bacteria which have the guar gel degradation ability to extract the thermostable enzyme as the breaker and to use bacteria to clean the residue of gel. Thermophilic enzyme has been the hotspot in the petroleum microbiology and using bacteria to clean the residue of gel is a completely novel concept to solve the blocking problem after hydraulic fracturing. Analysis of 16S rDNA sequence is used to identify the bacteria and viscosity of guar gum aqueous solution is used to assess the biodegradation ability of bacteria. One strain is identified as Geobacillus sp. T2. This strain is efficient in degrading guar gum at high temperature (65~70 oC). The reduction rate of the viscosity of guar gum solution is more than 90%. It means that this strain has the great potential to extract the thermophilic enzyme or to degradate the gel residue in oil and gas formation.

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