Health Care : Current Reviews

Identification of genes involved in biofilm formation of mycobacteria in relation to susceptibility of antimicrobial agents

Joint Event on 3^rd International Conference on Medical Sciences, Hypertension and Healthcare and World Congress on Organ Transplantation and Artificial Organs

August 24-25, 2018 Tokyo, Japan

Virendra Kumar

National JALMA Institute for Leprosy & Other Mycobacterial Diseases, India

Posters & Accepted Abstracts: Health Care Current Reviews

Abstract:

Bacterial biofilms are often associated with infections especially with medical implants such as catheters and other medical devices. Biofilms are formed to protect the bacteria from host defenses, antibiotics and from harsh environmental conditions. We had taken four mycobacterial species (M. smegmatis, M. fortuitum, M. avium and M. tuberculosis) for study of mycobacterial biofilm. The isolates were subculture and characterized biochemically and molecularly. The large quantity of biofilm was produced by M. smegmatis at temperature 37 oC and 42 oC as compared to 30 oC. M. fortuitum developed more amount of biofilm at 30 oC as compared to 37 oC and 42 oC. M. avium developed strong amount of biofilm at 30 oC and 42 oC as compared to 37 oC. M. tuberculosis (H37Rv) developed strong biofilm at 37 oC and no biofilm at 30 oC and 42 oC in MB7H9 media and Sauton media. The selected non-tuberculosis mycobacteria and H37Rv developed strong biofilm in the presence of OADC enrichment in MB7H9 as well as Sauton???s medium. Antibiotic susceptibility of biofilms at ultrastructural level was also studied in fast growing clinical isolates M smegmatis in presence of Streptomycin, Isoniazid, Rifampicin, Ethambutol and Pyrazinamide. Isoniazid showed strong inhibited biofilm in fast grower and sensitive isolates. However, Pyrazinamide and Isoniazid inhibited biofilm of M. tuberculosis (H37Rv) and in MDR isolates Ethionamide and Moxifloxacin inhibited biofilm in slow grower and fast grower mycobacteria. However, many mycobacterial species are known to form biofilms, little is known about the genetic requirements and patterns of gene expression. In micro array hybridization we have found that six genes were expressed in M. avium. In M. tuberculosis MDR isolates seven genes were expressed and two genes Rv0359 and Rv3526 were homologues as earlier reported in P. areuginosa and M. avium which might be responsible for biofilm formation.

Biography :

E-mail: vksjalma@gmail.com