Journal of Microbial & Biochemical Technology

Identification of Gardnerella vaginalis in bacterial vaginosis among symptomatic women in Duhok city, Kurdistan

International Conference and Expo on Water Microbiology & Novel Technologies

July 18-19, 2016 Chicago, USA

Ismaeil Mohammed Abdulkahar Mammani and Bland Husmuldeen Abdullah

Duhok University, Kurdistan

Posters & Accepted Abstracts: J Microb Biochem Technol

Abstract:

Gardnerella vaginalis bacteria; is considered the most relevant organism associated to Bacterial Vaginosis (BV), which is leading to vaginal disorders. The aim of this study was to apply the DNA sequencing technique for Gardnerella vaginalis strains and compare the strains obtained in this study with other international strains. PCR products of (15) G. vaginalis isolates that have been sent for sequencing all of them have been sequenced successfully and then aligned with the sequences in the GenBank database using BLAST program. The results of Mole-BLAST Tree were showed that the samples Bland_GVK_(14,6,3,10) were had similarity (99%) to G. vaginalis strain under the Accession number (AP012332.1/ USA), while the remain samples (1, 2, 5, 7, 8, 9, 11, 12, 13, 15 & 16), were found close to the strain of G. vaginalis with similarity (99%) under the Accession number (CP002725.1/Japan). Phylogenetic tree showed the similarity and evolutionary relationship between the sequenced samples and reference sequences in GenBank database. The Phylogenetic analysis also revealed that the genus G. vaginalis comprises a single species; shows G. vaginalis form a distinct clade within the Bifidobacteraceae family most closely related to Bifidobacterium scardovii.

Biography :

Email: ismaeil.mohammed@uod.ac