Pharmaceutica Analytica Acta

Hydrodynamic chromatography coupled to ICP-MS for studying nanoparticles in complex media

International Conference and Exhibition on Advances in HPLC & Chromatography Techniques

March 14-15, 2016 London, UK

Allan Philippe

Koblenz-Landau University, Germany

Scientific Tracks Abstracts: Pharm Anal Acta

Abstract:

Investigation of colloids behaviour in the environment are highly challenging due to the lack of robust and efficient methods for sizing and quantifying engineered or natural colloids in environmental media. Hydrodynamic chromatography (HDC) coupled with ICPMS is a practical and efficient technique for the detection of inorganic nanoparticles in complex media. The claimed advantages of the method are its versatility, its relative simplicity, high recoveries, and the possibility to combine sizing and quantitative analysis. Despite ist long history, HDC validation for environmental samples and complex particles has been investigated only recently. Especially, the effect of particle shape on the retention factor remained unaddressed, although most natural colloids are non-spherical. The recent development of single particle ICP-MS (SP-ICP-MS) made possible to use this technique in combination with HDC with a powerful synergy. This presentation reports and discusses our advances in the development of HDC-SP-ICP-MS to analyse metal containing nanoparticles (e.g. Au, Ag, TiO2, CeO2) in complex media. Using standard equipment and well characterized calibrants with different densities and coatings, we demonstrated the universality of the size calibration method for HDC-ICP-MS. Several applications of this technique to complex media demonstrate its versatility. Based on measurements of standard particles having different shapes, we propose a method to differentiate between spherical and non-spherical, including fractal agglomerates, using HDC-SP-ICP-MS. Powerful combination of detectors, versatility, and possibility to size and quantify inorganic colloids simultaneously in complex matrixes are strong arguments for a wide application of HDC in colloids analysis in environmental sciences.

Biography :

Allan Philippe has completed his PhD in 2015 at the Institute of Environmental Chemistry at Koblenz-Landau University on the interactions of natural organic matters and engineered nanoparticles and on the development of HDC-ICP-MS for environmental samples. His current focus are on the further development of analyitcal techniques for characterizing colloids in environmental matrices.

Email: philippe@uni-landau.de