Pharmaceutica Analytica Acta

High performance liquid chromatographic method for the pharmacokinetic study of saroglitazor formulation after injection to rats

International Conference and Exhibition on Advances in HPLC & Chromatography Techniques

March 14-15, 2016 London, UK

Rajendra B Kakde

Rashtrasant Tukadoji Maharaj Nagpur University, India

Posters & Accepted Abstracts: Pharm Anal Acta

Abstract:

A method based on solid phase extraction has been developed for the determination of saroglitazar in rat plasma after oral administration by high-performance liquid chromatography coupled with PDA detection (HPLCâ??PDA). Variables parameter affecting the solid phase extraction efficiency were evaluated and optimized. Chromatography separation was performed on a thermo hypersil C18 column (4.6 mm Ã? 250 mm, 5 μ) by isocratic elution with PDA detection at 279 nm. The assay was linear over the range of 4-20 μg/ml and the lower limit of quantification (LLOQ) was 4 μg/ml. The extraction recoveries were more than 77%, the accuracies were within 3.97%, and the intra- and inter-day precisions were less than 9.36% in all cases. After strict validation, the method indicated good performance in terms of reproducibility, specificity, linearity, precision and accuracy, and it was successfully applied to the pharmacokinetic study of saroglitazar in rats after oral administration.

Biography :

Email: sdrkakde@yahoo.com