Simeon Oluwatoyin Ayoola and Omoile Loveth Nkonye
University of Lagos, Nigeria
Posters & Accepted Abstracts: J Aquac Res Development
This study was carried out to evaluate the use of Tetracarpidium conophorum shells on the growth performance, its genotoxic effects, haematological and biochemical parameters of Clarias gariepinus juveniles for a period of twelve (12) weeks. Five experimental diets were formulated at 0% (control), 25% (T1), 50% (T2), 75% (T3) and 100% (T4) inclusion of walnut shell respectively. The experiment was carried out in plastic tanks, each treatment having three replicates. The fish in tank T1 (25% inclusion of T. conophorum) had the best weight gain with the mean of 33.5±5.8 g and the least was recorded in tank T4 (100% inclusion of T. conophorum). The specific growth rate was highest in tank T3 (75% inclusion of T. conophorum) with the mean value of 0.46±0.05 g was recorded. The highest feed intake was found in fish fed with 25% inclusion of T. conophorum. There was no significant difference in the growth performance of all the treatments. No mortality was recorded in all the experimental tanks. The fish fed with T. conophorum showed increased haematological values of haemoglobin, (12.05±1.63 g/ dL), red blood cell, (2.785±0.28 ?L) and white blood cell, (11.25±4.59 ?L) compared to the values of fish fed with control diet with red blood cell, (1.81±1.54 ?L) and white blood cell, (5.15±6.57 ?L). There was reduction in the haematological value of the fish fed with control feed having haemoglobin, (10.75±8.13 g/dL). The genotoxicity test that was carried out showed that the highest counts of micronucleus were in tank T3 (3057±312.33) while the lowest count of micronucleus was found in tank T0 (1501±346.5c). A significant difference was recorded (p<0.05). It was concluded that using T. conophorum shells as feed for Clarias gariepinus enhances the growth of the fish and has no negative impact on the health status of the fish. Therefore partial replacement of feed with T. conophorum should be encouraged.
E-mail: sayoola@unilag.edu.ng