Agrotechnology

Genetic relationship among cultivars (Carica papaya L.) and Wild Papaya (Vasconcellea species) using molecular markers

International Conference on Agricultural & Horticultural Sciences

September 14-15, 2012 Hyderabad International Convention Centre, India

Kanupriya C, Aswath, C, Dinesh, M.R, Vasugi, C, Radhika, V and Shobhana M

Scientific Tracks Abstracts: Agrotechnol

Abstract:

Two different molecular methods namely, Random Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeats (ISSR) were used to measure genetic diversity among thirteen commercially important cultivars of papaya and two species of useful wild papaya. Eleven ISSR and 16 RAPD primers were screened to amplify a total of 75 and 118 bands respectively. Of these, approximately 35% and 37.2% of the bands were polymorphic in each. Genetic similarity was computed using Windist module of Winboot Software and the results displayed graphically as Neighbour Joining trees. Both methods revealed different groupings of the thirteen cultivars. On the basis of correlation of data with morphological grouping based on fruit pulp colour and plant height, ISSR was judged to be best method for analyzing all papaya germplasm.