Journal of Stem Cell Research & Therapy

Gene therapy using in vivo electroporation for alveolar bone regeneration

4^th International Conference and Exhibition on Cell & Gene Therapy

August 10-12, 2015 London, UK

Mariko Kawai1,2

Posters-Accepted Abstracts: J Stem Cell Res Ther

Abstract:

Bone morphogenetic proteins (BMPs) have strong bone inductive potential. We constructed a new method for bone
regeneration using BMPs gene and in vivo electropopration. We transferred BMPs gene to mesenchymal stem cells in
the periodontal tissues using in vivo electroporation. We observed time course cell populations after BMPs gene transfer. We
performed histological analyses and immune-histochemical analyses using stem cell markers CD90, CD44 or inflammatory
marker, CD68. Moreover, we performed calcein and tetracycline double bone staining for observation of the alveolar bone
metabolism. We found inflammation reactions during 5 days after BMPs gene transfer. Inflammatory tissues were not found
after one week later gene transfer. Double bone staining after BMPs gene transfer had wider alveolar bone lining. But, we
could not find any other differences compared with control. Our histological findings did not reveal that BMPs gene transfer
to periodontal tissues of rats made wrong alveolar bone metabolism. Therefore, our BMPs gene transfer system using in vivo
electroporation could be new therapy for alveolar bone regeneration.

Biography :

Mariko Kawai is DDS. She has completed her PhD from Kyoto University, Medical School. She is working as an Assistant Professor at Okayama University and
Osaka Dental University, Japan