Agrotechnology

Gamma ray induced mutagenesis in Zinnia elegans and the characterization of mutants using RAPD markers

7^th Global Summit on Agriculture & Horticulture

October 17-19, 2016 Kuala Lumpur, Malaysia

Pallavi B, Shashikiran Nivas, L D′Souza, Ganapathi T R2 and Smitha Hegde

Mangalore University, India
Bhabha Atomic Research Centre (BARC), India

Posters & Accepted Abstracts: Agrotechnol

Abstract:

Gamma ray induced mutagenesis has been used to produce a large number of new and novel variations in ornamental plants. 70% of the worldâ??s mutant varieties have been induced through gamma rays. The objective of the study is to induce mutations in Zinnia elegans an important ornamental plant using gamma rays and to apply RAPD analysis for the detection of genetic polymorphisms among mutants and the parent plant. The morphological variations such as plant height, flower diameter and flower number and the variation in color and forms of the flowers of mutants and parent plant were observed. The analysis of variance of the results revealed significant variations in the morphological traits. RAPD markers have been increasingly used to analyze genetic variations in mutagenized populations of ornamental plants obtained through gamma ray induced mutagenesis. 10 random RAPD primers were used in the study. The banding patterns obtained were analyzed using Jaccardâ??s similarity coefficients and phylogenetic analysis. Similarity coefficients were found to be ranging from 0.58-0.89. The cluster analysis showed a high level of genetic diversity between parent and the mutants. In conclusion, the gamma ray irradiation can be successfully utilized to induce mutations in ornamentals which can be commercialized. RAPD technique could be applied successfully to differentiate between mutants and the parent plant.

Biography :

Pallavi B has completed her Master’s studies in Biotechnology at St. Aloysius College, Mangalore from Mangalore University, India. Currently, she is pursuing her Doctoral studies under Mangalore University. She has presented her work at 3 international seminars and 7 national level seminars.

Email: pallavi8659@gmail.com