Functional tricalcium-phosphate influence on remineralization process of fluoridated enamel surface
12th World Congress on Dentistry and Oral Health
August 04-05, 2016 Manchester, UK

Sherif Helal

Riyadh College of Dentistry and Pharmacy, KSA

Posters & Accepted Abstracts: Oral Health Dent Manag

Abstract:

Objective: Recently, manufacturers introduced fluoride varnishes with functional additives as tricalcium-phosphate (TCP), induced deep in enamel surface aiming hydrolysis and calcium hydroxyapatite with polymorph tri-calcium phosphate formation; evidenced to improve enamel resistance to demineralization, but little studies available regard whether such additives will enhance or hinder the original fluoride remineralization potentiality. Method: 37 sound extracted permanent premolars (for orthodontics reason) crowns were sectioned and divided mesiodistally into an area-A which received 5% sodium fluoride varnish with additive Tricalcium phosphate (3M ESPE Clinproâ?˘ Seefeld; Germany), area-B was control without any coating and area-C received 5% sodium fluoride varnish without additive (Fluorproptector®â?? IvoclarVivadent; Leichtenstein) coating. Teeth submitted for 5 days pH-cycle regime, blocks kept individually in demineralizing solution (2.0 mM calcium, 2.0 mM phosphate, 0.030 ppm F, in 75 mM acetate buffer, pH 4.3) for 3 h (20 ml per block) and in remineralizing solution (1.5 mM calcium, 0.9 mM phosphate, 150 mM of KCl, 0.05 ppm F in 20 mM cacodylate buffer, pH 7.4) for 20 h (10 ml per block). After each cycle, blocks were kept in artificial saliva solution. This cycle was repeated daily and blocks remained in remineralizing solution for 2 days till analyses. Samples were dried and examined with EDAX scanning microscope and 5 KV accelerating voltage at high vacuum and magnifications of 6000x. Results: One-way analysis of variance (ANOVA) showed a significant difference (p<0.05) with best results among FL+fTCP in remineralization efficiency (m=3.48 ±SD=0.426) followed by FL-fTCP (m=2.98 ±SD=0.419) and at last the control group (m=1.98 ±SD=0.410), typical SEM enamel presented 3 aspects 1) demineralized keyhole rodâ??s core with intact inter-rod areas, 2) intact enamel rods and prismatic substances, 3) remineralization displayed as thick and more frequent lines with no evidence of porosities or decalcification. Conclusion: Results suggest that, fTCP additive significantly improve remineralization capacity of fluoride preparations and there is a need for further clinical validation.

Biography :

Email: drsherifhelal@riyadh.edu.sa