Journal of Pharmacogenomics & Pharmacoproteomics

Functional analysis using metabolic substrates-induced in vitro model of cardiac metabolic syndrome

3^rd International Conference on Predictive, Preventive and Personalized Medicine & Molecular Diagnostics

September 01-03, 2015 Valencia, Spain

Yin Hua Zhang

Seoul National University, Korea

ScientificTracks Abstracts-Workshop: J Pharmacogenomics Pharmacoproteomics

Abstract:

Cardiac metabolism is essential in myocardial contraction. Here, we analyzed the effect of metabolic substrates (fatty acids, pyruvate and lactate in normal Tyrodeâ??s solution, termed NF) on myocyte contractility in rat left ventricular myocytes. Our results showed that NF significantly increased myocyte contraction and intracellular Ca2+ transients. L-type Ca2+ current or Na+- Ca2+ exchanger activity was not increased and myofilament Ca2+ sensitivity was reduced by NF, suggesting key role of myofilament on cardiac Ca2+ homeostasis and contraction with NF. Furthermore, NF diminished insulin-dependent tyrosine phosphorylations of insulin receptor or receptor substrate and eNOS-Ser1177. Beta-adrenergic stimulation with isoprenaline significantly increased spontaneous myocyte contraction during diastole in NF. Collectively, NF impairs insulin signaling and reduces bioavailability of eNOS-derived NO, which desensitizes myofilament Ca2+ sensitivity, increases Ca2+ level and contraction. In addition, it predisposes beta-adrenergic arrhythmogenesis in cardiac myocytes. The results reveal that it resembles an in vitro model of cardiac metabolic syndrome.

Biography :

Yin Hua Zhang has completed her PhD in 1999 from Seoul National University, College of Medicine and Post-doctoral studies from Oxford University, Cardiovascular Medicine. She directs Cardiovascular Laboratory studying Physiology and Pathology of the heart. She has published more than 40 papers in high ranking journals and is holding a number of national grants.

Email: yinzhang@snu.ac.kr