Journal of Nanomedicine & Nanotechnology

Fibrin functionalized Graphene oxide as an osteoinductive material

3^rd International Conference on Nanotek & Expo

December 02-04, 2013 Hampton Inn Tropicana, Las Vegas, NV, USA

T. P Sastry and R. Deepachitra

Accepted Abstracts: J Nanomed Nanotechnol

Abstract:

Surface functionalized graphene oxide (SFGO) nanoparticles find its wide biomedical applications such as biosensors, biomedicine, bio-imaging, drug delivery and biodetection. This versatile biomedical application of GO is due to its biochemical nature, which enables greater interactions with biomolecules such as proteins through covalent and non-covalent interactions. Apart from this, the honeycomb crystal structure of GO acts as a platform for the addition of various biomolecules, which form incredible GO biomaterials. The biological compatibility of SFGO is evaluated using in vitro studies and this is supported by conducting experiments such as biocompatibility and haemocompatibilty. Fibrin (F) is an insoluble protein, serves as an ideal substrate for cell attachment, proliferation, extracellular matrix formation in wound healing and as a biological scaffold in tissue engineering. The drugs/hydroxy apatite can also be coupled to the fibrin based nanomaterials through the ligands. The fibrin isolated from biological waste material is used to functionalize the GO to make it as a multifunctional therapeutic nanocarrier for its biomedical applications. Thus prepared SFGO nanoparticles is characterized using UV-visible spectroscopy, fourier transform infra-red spectroscopy, scanning electron microscopy, transmission electron microscopy and X-ray diffraction technique. The biological evaluation such as biocompatibility and osteoconductivity was carried out using 3-(4, 5-dimethylthiazol-2-Y)-2, 5-diphenyltetrazolium bromide and alkaline phosphatase assays using both normal (NIH 3T3) and cancer (MG 63) cell lines. The results reveal biocompatibility and osteoinductive of the SGFO. Based on this finding, SGFO might be tried as an osteoinductive material in small animals.

Biography :

T. P Sastry is presently working as Senior Principal Scientist and Head in Bio-products Lab, Central Leather Research Institute, Chennai, India. He has completed his Ph.D. in Chemistry and has thirty years of research experience in the processing of animal bio-products into biomaterials and reducing the environmental pollution due to tannery and slaughter house wastes. He has published more than 70 papers in national and int ernational journals and filed 17 patents. He has commercialized 6 technologies to the relevant industries and 2 technologies were commercialized to an international company. He is expert in preparation, characterization and evaluation of Nano Biomaterials used in wound dressing, ophthalmic, oral and orthopedic surgery.