Journal of Nanomedicine & Nanotechnology

Fabrication of PANI nanowire as an electrochemical biosensor for DNA detection

13^th International Conference on Nanotek & Expo

December 05-07, 2016 Phoenix, USA

Soha Mohajeri

Sharif University of Technology,Tehran, Iran

Posters & Accepted Abstracts: J Nanomed Nanotechnol

Abstract:

Polyaniline (PANI) is an organic polymer that possesses metallic conductivity and can be grown 1-dimensionally in the form of a nanowire. In this research the potentiostatic method was applied at the constant potential of 0.75 V to electrochemically deposit PANI nanowires on the stainless steel electrode. Morphological studies of the sample was carried out by Transmission Electron Microscopy (TEM) and it was observed that nanowires with 30â??50 nm diameters were fabricated. Cyclic Voltammetry (CV), Scanning Electron Microscopy (SEM), and UVâ??vis absorption spectra were applied to characterize the PANI nanowires, and the results revealed that ultra-thin nanowires displayed high electrochemical activity. A single stranded Deoxyribonucleic acid (ssDNA) was fixed on PANI nanowires to investigate the efficiency of the system as a biosensor platform. Sensitivity of the PANI electrode was detected by measuring peak currents in Differential Pulse Voltammetry (DPV) after hybridization with different concentrations of the ssDNA. It was concluded that the system worked well even at low concentrations, and large peak current values at the order of mA were produced. Electrochemical Impedance Spectroscopy (EIS) of the sensor electrode was carried out with an Autolab-30 potentiostat/galvanostat to understand chemical transformations and processes associated with conducting polymer supported electrodes. The changes in impedance indicated that the system was extremely effective at low (10â??16M) concentrations.

Biography :

Email: soha.mohajeri@yahoo.com