Journal of Stem Cell Research & Therapy

Extraction of blood mesenchymal stem cells with the low speed centrifugation concept: Applications in regenerative medicine

10^th Annual Conference on Stem Cell & Regenerative Medicine

October 08-09, 2018 | Zurich, Switzerland

Joseph Choukroun

Pain Clinic, France

Scientific Tracks Abstracts: J Stem Cell Res Ther

Abstract:

Regenerative therapy with stem cells has gained tremendous momentum over the past decade as a modality geared towards markedly improving wound healing of various tissues by utilizing undifferentiated autologous host cells. While stem cells may be isolated from various locations in the human body, more recently it has been shown that low levels of mesenchymal stem cells also exist circulating within peripheral blood. Platelet rich fibrin (PRF) is a regenerative modality that utilizes peripheral blood + centrifugation protocols without the use of anti-coagulants to create a three-dimensional tissue engineering scaffold containing both growth factors and autologous cells. Very recently, it has been shown that modifications to centrifugation speed and time following recently developed concepts (the low-speed centrifugation concept or LSCC) resulted in a marked increase in host cells and growth factors. Within these scaffold constructs, mesenchymal stems cells were also found following collection with this relatively painless and low-cost modality. The objective of the present talk will be to present recent modifications to centrifugation speed and time to optimize stem cell quantities within PRF. Thereafter, the biological data supporting their numbers, as well as their potential for clinical applications will be presented with data coming from many fields of medicine including for the regeneration of osteoarthritic knees, dental regenerative medicine, orthopedic grafting, and for facial esthetics.

Recent Publications

1. J Choukroun and S Ghanaati (2018) Reduction of relative centrifugation force within injectable platelet-rich-fibrin (PRF) concentrates advances patients??? own inflammatory cells, platelets and growth factors: the first introduction to the low speed centrifugation concept. European Journal of Trauma and Emergency Surgery 44(1):87-95.

2. Wang X, Zhang Y, Choukroun J, Ghanaati S and Miron R J (2018) Effects of an injectable platelet-rich fibrin on osteoblast behavior and bone tissue formation in comparison to platelet-rich plasma. Platelets 29(1):48-55.

3. El Bagdadi K, Kubesch A, Yu X, Al-Maawi S, Orlowska A, Dias A and Choukroun J (2017) Reduction of relative centrifugal forces increases growth factor release within solid platelet-rich-fibrin (PRF)-based matrices: a proof of concept of LSCC. European Journal of Trauma and Emergency Surgery 1???13.

4. Miron R J, Fujioka-Kobayashi M, Hernandez M, Kandalam U, Zhang Y, Ghanaati S and Choukroun J (2017) Injectable platelet rich fibrin (i-PRF): opportunities in regenerative dentistry? Clinical Oral Investigations 21(8):2619-2627.

Biography :

Joseph Choukroun completed MD from University of Montpellier, France 1979 and is a Specialist in General Surgery, Anesthesiology from the same university. He is also a Specialist in Pain Management from the University of Strasbourg, France. He is the Owner of Private Pain Clinic, Nice France. He is the President of SYFAC, international symposium on growth factors. He is the Inventor of the PRF techniques: L-PRF, A-PRF and i-PRF. He is a Researcher working in Form Lab at the University of Frankfurt. He is the Author of several scientific publications and is recognized as an International Speaker.

E-mail: joseph.choukroun@free.fr