Journal of Pharmacogenomics & Pharmacoproteomics

Expression of integrin receptors and their ligands in papillary thyroid carcinoma with different BRAF V600E mutation status

2^nd International Conference on Predictive, Preventive and Personalized Medicine & Molecular Diagnostics

November 03-05, 2014 Embassy Suites Las Vegas, USA

Galina P Logacheva, L A Mostovich, S V Svyatchenko, S P Shevchenko and L F Gulyaeva

Posters: J Pharmacogenomics Pharmacoproteomics

Abstract:

Papillary thyroid cancer (PTC) is the most common malignancy of the endocrine system. The most frequent genetic alteration in PTC is V600E mutation in BRAF gene which leads to changes in the activity of intracellular signaling pathways. As a result, expression levels of cell membrane integrin receptors and their ligands - extracellular matrix proteins - osteopontin (OPN) and thrombospondin-1 (TSP1) change. Such changes promote migration, invasion and metastasis of tumor cells. Thus, integrin receptors and their ligands are potential biomarkers of an aggressive PTC phenotype. The aim of our study was to compare the gene expression profile of integrins ITGA3, ITGAV, ITGA6, ITGA9, ITGB1 and their ligands OPNa, OPNb, and TSP1 in PTC with different BRAF V600E mutation status.Intraoperative thyroid tissue samples from 41 patients diagnosed with papillary thyroid carcinoma (n=26), diffuse nodular nontoxic goiter (n=10) and follicular adenoma (n=5) were analyzed to evaluate the expression levels of the investigated genes that had been determinedby real time RT-PCR. Immunohistochemistrywas usedto confirm the PCR results and to estimate the amount of protein products.The presence ofBRAF V600E mutation was identified using allele-specific amplification. In this study asignificant increase (p>0.05) in expression levelsof ITGA3, ITGAV, ITGB1, OPNb and TSP1was observed in the PTC tissue samples.The expression levels of ITGA3 and ITGAV were higher in the BRAF V600Epositive PTC samples. The observed changes in the expression levels of the studied genes indicate their potential role in tumor progression and the possible impact on their expression of the mutant product of gene BRAF.Integrins and their ligands OPN and TSP1 can be considered to be potential markers in determining the prognosis and treatment of PTC.

Biography :

Galina P Logacheva graduated from Medical department of Novosibirsk State University (NSU) in July 2014. Since September 2014, she has been a clinical intern in Oncology on the base of NSU and a junior doctor of the head and neck tumor department in Novosibirsk Clinical Hospital 1. She is also engaged in research work and has participated in student and oncological conferences.