Neeti Sanan Mishra
International Center for Genetic Engineering and Biotechnology, India
Posters-Accepted Abstracts: J Food Process Technol
The microRNAs (miRNAs) are recognized as key modulators of gene expression in plant growth and development. Increasing evidence supports the fact that miRNAs act as genetic buffers in plant gene regulation providing protection against various abiotic and biotic stress conditions. We adopted the approach of Next Generation Sequencing (Illumina) technology for comparative profiling of miRNAs under various stress physiologies across different tissues in rice varieties. This revealed the expression patterns of several known and novel miRNAs. The expression patterns of selected stress deregulated miRNAs were experimentally validated. The targets of these miRNAs were also predicted and validated. Time kinetics studies helped to capture the narrow windows of miRNA target correlations indicating their role in tissue development. This data was compiled and developed as a web server, ARMOUR, which is now being used to select miRNAs for functional analysis. The studies have indicated novel outcomes that can be utilized to prepare ??smart plants? which will be an enduring step to fight against abiotic stresses mediated decline in crop yields in rice.
Email: neeti@icgeb.res.in