Fermentation Technology

Exploiting 3rd generation sequencing reads for de novo assembly of bacterial genomes

World Congress on Beneficial Microbes: Food, Pharma, Aqua & Beverages Industry

August 25-27, 2015 Valencia, Spain

Stephane Duboux, Deborah Moine, Gregory Lefebvre and Patrick Descombes

Nestle Institute of Health Sciences, Switzerland
Nestle Research Center, Switzerland

Posters-Accepted Abstracts: Ferment Technol

Abstract:

Recent studies are pointing towards ever increasing and new roles for microorganisms in numerous situations including human health, food optimization processes, pathogens infections etc. Complete high quality genomic information is a prerogative to the comprehensive analysis of these microorganisms and the subsequent association of genotypes to phenotypes. De novo genome assembly is a complex process directly influenced by the structure (length and number of repeats and transposable elements) and the GC composition of the genome. We are presenting here our complete workflow for de novo assembly of bacterial genomes, which includes a combination of various laboratory procedures, sequencing steps and data analysis, with examples of various bacterial strains of different genome size, GC contents and genome structure characteristics.

Biography :

Stephane Duboux is currently working as a researcher in Nestle Research Center, Switzerland.

Email: stephane.duboux@rdls.nestle.com